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C. S. Siva Prasath ¹, Geo S. M. Anishka ², S. Prakash ²

Affiliations
1 Department of Biotechnology, Udaya School of Engineering, Ammandivilai, Kanyakumari (T.N.), IN
2 Department of Biotechnology, Udaya School of Engineering, Udaya Nagar, Ammandiviali, Kanyakumari (T.N.), IN

Abstract

The main purpose of these works is to reveal antimicrobial activity of bacteriocin produced by Lactobacillus species. It has large spectrum of inhibition against photogenic food spoilage microorganisms etc., Lactobacilli are important organisms recognized for their fermentative ability as well as their health and nutritional benefits. They produce various compounds such as organic acids, diacetyl, hydrogen peroxide and bacteriocin or bactericidal protein during lactic fermentation. Lactobacilli was cultured in MRS broth and incubated for 24 hrs. After incubation the broth was centrifuged and supernatant was collected. The antimicrobial activity of the bacteriocin was assayed by agar spot test using gram positive and gram negative bacteria. The thermo stability of the antibacterial activity was determined and assayed for bacteriocin. The antimicrobial substance produced by the strain was thermo resistant for 30min at 100°C. Maximum activity in composed medium was achieved at initial pH of 5.5 and incubation period of 48hrs at 30-37°C. The supernatant was subjected to UV treatment by exposing the supernatant to UV light. Plasmid was isolated using STE buffer and no plasmids were suggesting that the genes encoding bacteriocin production were located on the genome.

Keywords

Bacteriocin, Lactobacilli, Antimicrobials.

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Geo S. M. Anishka ¹, C. S. Siva Prasath ¹, S. Prakash ¹

Affiliations
1 Department of Biotechnology, Udaya School of Engineering, Kanyakumari (T.N.), IN

Abstract

The objective of this work was to study the B2 medium for the pertussis vaccine production and with the different concentration of Bactocasamino acid, Starch and Yeast extract concentrations in B2 medium for the effective production of vaccine. Studied the opacity and pH changes occurred due to the variations of these components in the B2 medium. The different concentrations of components were studied out and superiority of the medium medium has been consistently observed.

Keywords

Bordetella pertussi, B2 Medium.

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C. S. Siva Prasath ¹, S. Prakash ¹

Affiliations
1 Department of Biotechnology, Udaya School of Engineering, Udaya Nagar, Ammandivilai, Kanyakumari (T.N.), IN

Abstract

Optimizations of fermentation medium for Clostridium tetani (MTCC 449) in the effective production of tetanus toxin were studied. Modified Muller Media with different concentration of Meat infusion has been used for the production of tetanus toxin . In this present work, the comparative study was carried out to determine the efficacy of tetanus toxin with the commercially prepared meat infusion broth compared and the meat infusion powder supplemented in Muller medium. The observed results showed the higher titer value of toxin in Muller media supplemented with meat infusion with higher concentration. The OD of the cell growth and pH changes due to buffering capacity were also studied. The better growth of the C.tetani was observed in the MM medium which contain higher rate of meat infusion broth. The level of the tetanus toxin was measured by Lf test. Minimal lethal dose was performed to check the presence of tetanus toxin.

Keywords

Tetanus Toxin, Clostridium tetani, Fermentation Medium.

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